Laboratory of Systems Physiology and Clinical Exercise Physiology Laboratory

Laboratory of Systems Physiology

The Laboratory of Systems Physiology within the Department of Kinesiology occupies approximately 1800 ft2 of dedicated space and is designed for basic and applied research.  Primarily, this laboratory supports in vivo (both rodent and human), in vitro and molecular biology research with a systems physiology approach with the following major equipment:  

  • Tissue homogenizer
  • Eppendorf Mastercycler Gradient Thermal Cycler
  • Cryostat-microtome
  • Cryo-Pro cryogenic storage system
  • NanoDrop™ Spectrophometer
  • microplate reader
  • 5 4°C/ -20°C refrigerators/freezers
  • 3 x - 86°C sub freezers
  • Allegra X-22R refrigerated centrifuge
  • BioRad Mini-Protean II kit and Criterion XT kit for SDS PAGE with western blot analysis
  • IX71 inverted fluorescent microscope (Olympus)
  • Diagnostic ultrasound (Hitachi)
  • Isokinetic dynamometer (Biodex)
  • Noraxon force and EMG recording system
  • Custom micromechanical rig and microscope 

The Laboratory of Systems Physiology also contains a dedicated cell culture room that contains the following equipment: 

  • 4ft biological safety cabinet (Class II laminar flow)
  • Automatic water-jacket CO2 incubator
  • Inverted microscope  

The Laboratory of Systems Physiology occupies space in the University vivarium, which is equipped with: 

  • Conscious small rodent cardiopulmonary assessment systems (DSI and Buxco)
  • Aurora Scientific 1305A Muscle Test System
  • VistaVision Microscope with gooseneck and 150W fiber optic light source
  • Rodent treadmill with a metabolic gas analyzer designed for use on small rodents, (Oxymax system)
  • Philips Ultrasound/echocardiograph
  • Lunar PIXImus densitometer 

Common core facilities available to the Laboratory of Systems Physiology include, in part, The DNA Microarray Facility of the Charlotte Genomics Consortium.  The DNA Microarray Facility includes:

  • Model 640 GeneChip Hybridization Oven
  • Model 400 GeneChip Fluidics Station
  • Model 3000 GeneArray Large-Format Scanner
  • Two Total Desktop Mining Solution workstations consisting of Dell OptiPlex GX110 computers running Affymetrix Microarray Suite 5.0, MicroDB 3.0, and Data Mining Tool 3.0 software.  One workstation is located in the Cannon Research Center of the Carolinas Medical Center. The other is located in the Department of Biology at the University of North Carolina at Charlotte and is freely accessible to the Investigators.

 

Clinical Exercise Physiology Laboratory

The Department of Kinesiology also boasts a separate Clinical Exercise Physiology Laboratory, occupying 2500 ft2, which is well equipped to perform human research in this area.  Equipment in this lab includes:

  • 4 motorized treadmills (Trackmaster, Quinton and GE)
  • 5 Monark cycle ergometers
  • 1 Velotron cycle ergometer and accompanying computer set-up
  • 2 ParvoMedics metabolic carts
  • 3 CosMed metabolic carts
  • Blood pressure assessment equipment (sphygmomanometers, stethoscopes, cuffs)
  • 1 BodPod for body composition assessment
  • 1 Physician’s scale
  • 2 Schwinn Airdyne cycle ergometers
  • Multiple skinfold calipers and Gulick measuring tapes
  • Polar heart rate monitors
  • Hydrostatic weighing equipment
  • 1 hand-held blood lactate analyzer
  • 5 Nonin finger pulse oximeters for oxygen saturation assessment
  • 4 glucometers
  • Ankle-brachial testing equipment for ABI assessment
  • Sit-and-reach boxes, yardsticks, and goniometers for flexibility assessment
  • 5 I-Works metabolic stations for measurement of multiple physiologic parameters Including EMG, ECG and pulmonary function
  • 1 Quinton ECG stress testing system
  • 1 GE ECG stress testing system

This and other available laboratory equipment (and the supplies that goes with each piece of equipment) enables teaching for large groups of undergraduate and graduate students as well as human physiologic research.

Research Laboratory Faculty and Staff

Dr. Reuben Howden

Dr. Trudy Moore-Harrison

Dr. Susan K. Tsivitse

Dr. Michael J. Turner

Dr. Scott E. Gordon